Reagent Diagnostic Test Kits a Covid-2019 Zimatengera Real-time Pcr Platform

Indirect immunoenzyme assay to test antibodies to COV-2 in human serum/plasma.Complete solution IgG ndi IgM + IgA kits zilipo.Kuphatikiza kwa IgM + IgA kumathandizira kwambiri kukhudzidwa kwa kit.Common protocol imalola kuchita IgG ndi IgM + IgA simoltaneously in the same run.Yoyenera ku automated ELISA systems.Njira ya ELISA imachokera pa zomwe ma antibodies mu chitsanzo choyesedwa ndi antigen adsorbed pamtunda wa polystyrene. Ma immunoglobulins osamangika amatsukidwa. Ma enzyme otchedwa anti- human globulin amamanga antigenantibody complex mu sitepe yachiwiri. Pambuyo pa sitepe yatsopano yosamba, conjugate yomangidwa imapangidwa mothandizidwa ndi njira yapansi panthaka (TMB) kuti ipereke mankhwala osungunuka amtundu wa buluu omwe amasanduka achikasu pambuyo powonjezera njira yoyimitsa asidi• Kuchita kwakukulu ndi kukhazikika kotsimikizika ndi lyophilized conjugates pakafunika. â Zitsanzo ndi maulamuliro amakonzedwanso mofanana kuti alipire kusiyana kwa mapaipi.• Mabala amitundu okhala ndi zitsime zosweka.• Ma reagents amitundu, okonzeka kugwiritsa ntchito.

Zida zothetsera IgG ndi IgM+IgA zilipo.

Kuphatikiza kwa IgM + IgA kumakulitsa chidwi cha zida.

Protocol yodziwika bwino imalola kuchita IgG ndi IgM + IgA munthawi yomweyo.

Oyenera makina amtundu wa ELISA.

Njira ya ELISA imatengera momwe ma antibodies amachitira pachitsanzo choyesedwa ndi antigen adsorbed pamtunda wa polystyrene. Ma immunoglobulins osamangika amatsukidwa. Ma enzyme otchedwa anti- human globulin amamanga antigenantibody complex mu sitepe yachiwiri. Pambuyo pochapira chatsopano, conjugate yomangidwa imapangidwa mothandizidwa ndi substrate solution (TMB) kuti ipangitse chinthu chosungunuka chamtundu wa buluu chomwe chimasanduka chikasu pambuyo powonjezera njira yoyimitsa asidi.

• Kuchita kwakukulu komanso kukhazikika kotsimikizika ndi ma conjugates a lyophilized pakafunika.

• Zitsanzo ndi maulamuliro amakonzedwa mofanana kuti alipire kusiyana kwa pipeting.

• Ma mbale okhala ndi mitundu yokhala ndi zitsime zosweka.

• Zida zamadzimadzi zamitundu, zokonzeka kugwiritsa ntchito.